Responsibilities include:

• Perform cell culture and associated analytical assay activities for monoclonal antibody, recombinant protein and viral vector production in batch, fed-batch and perfusion modes using mammalian cell lines in shake flasks, stirred tank and single-use bioreactors.
• Execute protocols and studies to generate application data related to filtration-based perfusion cell culture to support existing technologies and new product development.
• Trouble-shoot cell culture processes by monitoring cell growth, viability, productivity and other cell culture metabolites.
• Issue internal scientific reports and publish articles in peer reviewed and trade journals. Present talks and posters at scientific conferences.
• Actively participate in expanding Repligen’s IP in the field of filtration-based perfusion cell culture applications by submitting invention disclosures.
• Work with other departments including product management, field applications and sales to accomplish individual and company objectives.

Minimum Requirements: 

• B.S Degree in chemical engineering, biological sciences, or applicable field with at least 5 years or MS with at least 3 years of industrial experience in a process or application development setting.
• Extensive understanding in biological process sciences, upstream process development including aseptic techniques, and cell culture processes.
• Experienced hands-on work setting up lab (2-10L) and pilot (50-200L) scale bioreactors, cultivating cells, and optimizing feeding and perfusion strategies.
• Experience in monoclonal antibody and recombinant protein upstream process development for therapeutic or vaccine applications.
• Demonstrated experience in design of experiments (DoE), strong analytical and problem-solving skills.
• Ability to work independently and within multidisciplinary project teams.
• Excellent oral and written communication abilities and strong interpersonal skills to communicate with internal and external team members.

Preferred Additional Qualifications:

• Experience with ATF technology.
• Experience in viral vector (AAV, Adenovirus, Lentivirus) upstream process development.
• Experience with perfusion cell culture using ATF technology is recommended.
• Understanding of membrane-based filtration including hollow fibers, determining critical flux, shear and TMP measurements.